Recurrence rate and progression of chondrosarcoma is correlated with heat shock protein expression
نویسندگان
چکیده
Heat shock proteins (HSPs) are involved in tumour immunity, and are correlated with survival and drug resistance in numerous types of cancer. The present study investigated the expression of HSPs and multiple drug resistance (MDR) in human chondrosarcoma. HSP and P-glycoprotein (the MDR1 gene product) expression was evaluated by immunohistochemical analysis of paraffin-embedded sections obtained from 37 patients with chondrosarcoma (19 male and 18 female; aged 33-85 years; mean age, 48.5 years). HSP73 and 90 were significantly overexpressed in patients with local recurrence: HSP73 was expressed in 7/7 patients (100%) with local recurrence and 9/18 patients (50%) without recurrence (P<0.02), while HSP90 was expressed in all patients with recurrence but only 8/18 (44%) without recurrence (P<0.02). A marked association was also identified between HSP expression and survival. HSP72 and 73 were significantly overexpressed in tumours from patients who succumbed to the disease (all positive for HSP72 and 73; P<0.05). No differences were observed between HSP27, 73 or 90-positive or -negative tumours according to age or gender. In addition, HSP72 expression was correlated with differentiation of the tumours (P<0.02). These results indicate that HSP72, 73 and 90 may function as novel prognostic markers for chondrosarcoma, and initiate further studies regarding the use of such markers for the identification of patients with poor prognosis.
منابع مشابه
Expression of Recombinant Heat-Shock Protein 70 of MCAN/IR/96/LON-49, a Tool for Diagnosis and Future Vaccine Research
Background: Heat shock protein 70 (HSP70) is present in all organisms studied so far, and is a major immunogen in infections caused by pathogens including Leishmania spp. Objective: The aim of this study was to clone and express HSP70 from L. infantum strain MCAN/IR/96/LON-49 and evaluate antibody response against HSP70 in visceral leishmaniasis (VL). Methods: The L. infantum HSP70 gene segment...
متن کاملPain Relief with Wet Cupping Therapy in Rats is Mediated by Heat Shock Protein 70 and ß-Endorphin
Background: Wet cupping therapy is a complementary therapy in pain management. The mechanism of this therapy, however, needs further elucidation. Cells injured by wet cupping therapy seem to stimulate the expression of heat shock protein 70 (HSP70). Its benefit in pain reduction could be mediated by the expression of ß-endorphin. This study aimed at determining the correlation between HSP70 and...
متن کاملP-107: The Effects of Cryotop Vitrification on Heat Shock Protein 72 Expression in Mouse 2-Cell Embryos by Nested Quantitative PCR
Background: The aim of the study was to compare the effects of two different concentrations of cryoprotectants by Cryotop vitrification on survival and Heat shock protein 72 (Hspa1a) expression of two-cell mouse embryos. Materials and Methods: Different cryoprotectants’ concentrations of the combination of dimethyl sulfoxide (DMSO) and ethylene glycol (EG) were used and compared with each other...
متن کاملP-11: Evaluation of Heat Shock Protein A2 in Male Rats before and after Varicocele Induction
Background: Varicocele is a major cause of male infertility, as it may impair spermatogenesis through several distinct pathophysiological mechanisms including hypoxia, renal-adrenal reflux, hormonal dysfunction, autoimmunity, oxidative stress and hyperthermia. HSP70s are members of the heat shock protein (HSP) family, which are considered intracellular chaperones. Their expressions can increase...
متن کاملCloning and Expression of Heat Shock Protein 60kDa Gene from Brucella melitensis as Subunit Vaccine
Brucellosis is caused by the bacterium Brucella and affects various domestic and wild species. GroEL (Heat Shock Protein 60kDa) as one of the major antigens that stimulate the immune system, increases Brucella survival. The aim of the current study was to clone and express GroEL in Escherichia coli in order to design subunit vaccine. Amplifying was performed using specific primers. The full-len...
متن کامل